Skip to main content

Human BMP-2/BMP-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3552

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
MAB3552-100
MAB3552-500
MAB3552-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Avian - Chicken

Applications

Validated:

Neutralization, Western Blot

Cited:

Bioassay, Cell Culture, Immunohistochemistry, Immunoprecipitation, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 100230

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP-2
Gln283-Arg396
Accession # P12643

Specificity

Detects human BMP-2 and human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant zebra fish (rzf) BMP-2 and rhBMP-4, 20%-40% cross-reactivity with rhBMP-3, -3b, -6, and no cross-reactivity with rhBMP-5, -7, -10, or -15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human BMP-2/BMP-4 Antibody

Alkaline Phosphatase Production Induced by BMP‑2 and Neutralization by Human BMP-2/BMP-4 Antibody.

Alkaline Phosphatase Production Induced by BMP‑2 and Neutralization by Human BMP-2/BMP-4 Antibody.

Recombinant Human BMP-2 (Catalog # 355-BM) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Human BMP-2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-2/BMP-4 Monoclonal Antibody (Catalog # MAB3552). The ND50 is typically 5-15 µg/mL in the presence of heparin (2 µg/mL).

Applications for Human BMP-2/BMP-4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human BMP-2 (Catalog # 355-BM)
Recombinant Human BMP-4 (Catalog # 314-BP)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize BMP‑2-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑2 and 2 µg/mL heparin. This antibody also neutralizes Human BMP-4 induced activity in the ATDC5 mouse chondrogenic cell line. The ND50 is approximately 3 μg/mL in the presence of 75 ng/mL of Recombinant Human BMP-4.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB3552 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2/BMP-4

Bone Morphogenetic Protein 2 (BMP-2) and BMP-4 are two of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.

Long Name

Bone Morphogenetic Protein 2/4

Alternate Names

BDA2, BMP-2, BMP2A, BMP-2A, BMP2ABone morphogenetic protein 2A, bone morphogenetic protein 2

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Gene Symbol

BMP2

UniProt

Additional BMP-2/BMP-4 Products

Product Documents for Human BMP-2/BMP-4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BMP-2/BMP-4 Antibody

For research use only

Loading...
Loading...
Loading...
Loading...
Loading...