Human BMP-2/BMP-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3552
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Avian - Chicken
Applications
Validated:
Neutralization, Western Blot
Cited:
Bioassay, Cell Culture, Immunohistochemistry, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 100230
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP-2
Gln283-Arg396
Accession # P12643
Gln283-Arg396
Accession # P12643
Specificity
Detects human BMP-2 and human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant zebra fish (rzf) BMP-2 and rhBMP-4, 20%-40% cross-reactivity with rhBMP-3, -3b, -6, and no cross-reactivity with rhBMP-5, -7, -10, or -15 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human BMP-2/BMP-4 Antibody
Alkaline Phosphatase Production Induced by BMP‑2 and Neutralization by Human BMP-2/BMP-4 Antibody.
Recombinant Human BMP-2 (Catalog # 355-BM) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Human BMP-2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-2/BMP-4 Monoclonal Antibody (Catalog # MAB3552). The ND50 is typically 5-15 µg/mL in the presence of heparin (2 µg/mL).Applications for Human BMP-2/BMP-4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human BMP-2 (Catalog # 355-BM)
Recombinant Human BMP-4 (Catalog # 314-BP)
under non-reducing conditions only
Sample: Recombinant Human BMP-2 (Catalog # 355-BM)
Recombinant Human BMP-4 (Catalog # 314-BP)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize BMP‑2-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑2 and 2 µg/mL heparin. This antibody also neutralizes Human BMP-4 induced activity in the ATDC5 mouse chondrogenic cell line. The ND50 is approximately 3 μg/mL in the presence of 75 ng/mL of Recombinant Human BMP-4.
Reviewed Applications
Read 2 reviews rated 4 using MAB3552 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-2/BMP-4
Bone Morphogenetic Protein 2 (BMP-2) and BMP-4 are two of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.
Long Name
Bone Morphogenetic Protein 2/4
Alternate Names
BDA2, BMP-2, BMP2A, BMP-2A, BMP2ABone morphogenetic protein 2A, bone morphogenetic protein 2
Gene Symbol
BMP2
UniProt
Additional BMP-2/BMP-4 Products
Product Documents for Human BMP-2/BMP-4 Antibody
Product Specific Notices for Human BMP-2/BMP-4 Antibody
For research use only
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