Human Bora Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB68561

R&D Systems, part of Bio-Techne
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MAB68561
MAB68561-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 694746

View all Bora Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bora
Gly2-Ala180
Accession # Q6PGQ7

Specificity

Detects human Bora in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Bora Antibody

Detection of Human Bora antibody by Western Blot.

Detection of Human Bora by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Bora Monoclonal Antibody (Catalog # MAB68561) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bora at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Bora Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bora

Bora (Aurora borealis; also C13orf34) is a 61 kDa member of the Bora family of proteins.  It is ubiquitously expressed, and plays a key role in cell cycle progression.  Plk1 (polo-like kinase-1) is a phosphorylase that is important to the cell during the G2/M transition and mitosis.  Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on Thr210.  Bora, Aurora-A and Plk1 all appear to form a complex during G2.  Bora predisposes Plk1 to the actions of Aurora-A.  Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora.  This initiates BORA dissociation from Aurora-A with subsequent ubiquitination and degradation.  Human Bora is 559 amino acids (aa) in length. It contains a Ser-rich region (aa 188-278) and at least eight utilized Ser phosphorylation sites.  Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85 kDa.  There is one potential alternative start site that lies 60 aa upstream of the standard start site, and a second splice variant the shows a 17 aa substitution for aa 1-87.  Over aa 2-180, human Bora shares 84% aa identity with mouse Bora.

Long Name

Protein Aurora Borealis

Alternate Names

Aurora Borealis, C13orf34

Entrez Gene IDs

79866 (Human); 777441 (Mouse); 306102 (Rat)

Gene Symbol

BORA

UniProt

Q6PGQ7 (Human)

Additional Bora Products

Product Documents for Human Bora Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Bora Antibody

For research use only

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