Human BTK Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11593
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3005A
Product Specifications
Immunogen
E. coli-derived recombinant human BTK
Glu280-Val377
Accession # Q06187
Glu280-Val377
Accession # Q06187
Specificity
Detects recombinant human BTK-SH2 in Direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human BTK Antibody
Detection of Human BTK by Western Blot.
Western Blot shows lysates of Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human BTK Monoclonal Antibody (Catalog # MAB11593) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTK at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human BTK Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTK
Long Name
Bruton Agammaglobulinemia Tyrosine Kinase
Alternate Names
AGMX1, IMD1, PSCTK1, XLA
Entrez Gene IDs
695 (Human)
Gene Symbol
BTK
UniProt
Additional BTK Products
Product Documents for Human BTK Antibody
Product Specific Notices for Human BTK Antibody
For research use only
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