Human c-Maf Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8227
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 883926
Product Specifications
Immunogen
E. coli-derived recombinant human c-Maf
Met1-Phe372
Accession # O75444
Met1-Phe372
Accession # O75444
Specificity
Detects human c-Maf in direct ELISA and Western Blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human c-Maf Antibody
Detection of Human c-Maf by Western Blot.
Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. Gels were loaded with 40 µg of cytoplasmic (Cyto) and 20 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Maf Monoclonal Antibody (Catalog # MAB8227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for c-Maf at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
c-Maf in RPMI 8226 Human Cell Line.
c-Maf was detected in immersion fixed RPMI 8226 human multiple myeloma cell line using Mouse Anti-Human c-Maf Monoclonal Antibody (Catalog # MAB8227) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human c-Maf Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed RPMI 8226 human multiple myeloma cell line
Sample: Immersion fixed RPMI 8226 human multiple myeloma cell line
Western Blot
2 µg/mL
Sample: RPMI 8226 human multiple myeloma cell line
Sample: RPMI 8226 human multiple myeloma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3.5 using MAB8227 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: c-Maf
Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian)
Alternate Names
CCA4, cMaf, MAF2
Gene Symbol
MAF
UniProt
Additional c-Maf Products
Product Documents for Human c-Maf Antibody
Product Specific Notices for Human c-Maf Antibody
For research use only
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