Human C-Peptide Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB80561
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 915612
Product Specifications
Immunogen
Human C-Peptide synthetic peptide
EAEDLQVGQVELGGGPGAGSLQPLALEGSLQ
Accession # P01308
EAEDLQVGQVELGGGPGAGSLQPLALEGSLQ
Accession # P01308
Specificity
Detects human C-Peptide in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human C-Peptide Antibody
C-Peptide in Human Pancreas.
C-Peptide was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB80561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human C-Peptide Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C-Peptide
Insulin is a peptide hormone that facilitates the cellular uptake of glucose by regulating the appearance of membrane glucose transporters. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 25-54), a C-peptide (aa 55-89), and a 21 aa A chain (aa 90-110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfide-linked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes. The human Insulin C-peptide shares 61% and 68% aa sequence identity with mouse and rat Insulin C-peptide, respectively.
Long Name
Proinsulin C-Peptide
Alternate Names
C-P, Insulin C-Peptide, Proinsulin C-Peptide
Gene Symbol
INS
UniProt
Additional C-Peptide Products
Product Documents for Human C-Peptide Antibody
Product Specific Notices for Human C-Peptide Antibody
For research use only
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