Human C1qR1/CD93 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2379
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
ELISA (detection), ELISA Development, ELISA Development (Detection), Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human C1q R1/CD93
Ala24-Lys580
Accession # Q9NPY3
Ala24-Lys580
Accession # Q9NPY3
Specificity
Detects human C1q R1/CD93 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse C1q R1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human C1qR1/CD93 Antibody
C1qR1/CD93 in U937 Human Cell Line.
C1qR1/CD93 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human C1qR1/CD93 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2379) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human C1qR1/CD93 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood monocytes
Sample: Human whole blood monocytes
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human C1q R1/CD93
Sample: Recombinant Human C1q R1/CD93
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C1qR1/CD93
References
-
Kim, T.S. et al. (2000) Mol. Immunol. 37:377.
-
Dean, Y.D. and P. Gasque (2001) Eur. J. Immunol. 31:1370.
Long Name
Complement Component C1q Receptor
Alternate Names
AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68
Gene Symbol
CD93
UniProt
Additional C1qR1/CD93 Products
Product Documents for Human C1qR1/CD93 Antibody
Product Specific Notices for Human C1qR1/CD93 Antibody
For research use only
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