Human C4.4A/LYPD3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5428
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human C4.4A/LYPD3
Leu31-His286
Accession # O95274
Leu31-His286
Accession # O95274
Specificity
Detects human C4.4A/LYPD3 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human C4.4A/LYPD3 Antibody
Detection of Human C4.4A/LYPD3 by Western Blot.
Western blot shows lysates of SK-BR-3 human breast cancer cell line, MCF-7 human breast cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for C4.4A/LYPD3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human C4.4A/LYPD3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: SK‑BR‑3 human breast cancer cell line, MCF‑7 human breast cancer cell line, and RT-4 human bladder carcinoma cell line
Sample: SK‑BR‑3 human breast cancer cell line, MCF‑7 human breast cancer cell line, and RT-4 human bladder carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C4.4A/LYPD3
LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI anchored metastasis-associated protein C4.4A) is an 85‑95 kDa, variably glycosylated member of the Ly6 family of GPI anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin‑3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296 amino acid (aa) GPI linked glycoprotein. It contains two UPAR domains
(aa 33‑126 and 140‑222), and an ADAM cleavage site (aa 312‑325). The molecule shows extensive N and O linked glycosylation. There is one potential isoform that shows a deletion of aa 129‑180. Over aa 31‑286, human LYPD3 shares 81% aa identity with mouse LYPD3.
Long Name
Leucine Rich Repeat and Fibronectin Type III Domain Containing 3
Alternate Names
LYPD3, MIG-C4
Gene Symbol
LYPD3
UniProt
Additional C4.4A/LYPD3 Products
Product Documents for Human C4.4A/LYPD3 Antibody
Product Specific Notices for Human C4.4A/LYPD3 Antibody
For research use only
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