Human Cadherin-11 Antibody

Detection of Human Cadherin-11 by Western Blot

PEDF overexpression promoted the MET-like behavior in pulmonary metastasis. (A–K, M) The results of higher doses (2 x 106 cells) injections into the knee joints. (A)In vivo luciferase-imaging of 143B/143B+PEDF cells after 28 days from the injection. The signals were observed in the lung of 143B+PEDF cell-injected mice (right, six positive/10: one dead), while no signal was seen in thorasic region of 143B-injected mice (left, zero positive/nine). (B, C) The H&E stain of knee joint and femur section. The scale bar indicates 1mm. (B’, C’) SPARC-IHC of the adjacent section of (B, C). The scale bar indicates 1 mm. (B) The 143B cells (ca; arrow) were mainly observed outside of the knee bone. (B’) SPARC-IHC. The cells outside of the joint were osteosarcomas. (C) 143B+PEDF cells were observed inside of the bone (ca; arrow). (C’) SPARC-IHC of (C). The SPARC-positive cancer cells were seen both in the bone marrow (bm) and the joint. The opened arrowheads mean the position of the bone. (D) The H&E stain of the pulmonary metastatic lesion of the 143B+PEDF-injected mouse. The square of the broken line is the region of interest in (E–G). (E–G) The lung vascular border in 143B+PEDF cell-injected mouse at higher magnification. The cancer cells inside and outside the vasculature are connected with each other. The scale bar indicates 50 µm. (E) shows H&E staining of these sections. (F) shows IHC for CD31 on a similar section. The endothelial cell layer is very thin. (G) shows IHC for PEDF. PEDF-positive cells are present both inside and outside the vein. (H–K, M) The 143B+PEDF tumor in the lung at higher magnification. The scale bar indicates 50 µm. (H) shows H&E staining of the inside and outside of vasculature. (I) shows IHC for CD31 indicating the endothelial layer. (J) shows IHC for beta-catenin on both sides of the boundary. (K) shows IHC for PEDF also on both the sides. (L) IHC for beta-catenin-in the normal bronchial tube near the tumor lesion. (M) IHC for beta-catenin in the tumor far away from the vascular boundary. Arrowheads indicate strong staining on cell–cell junctions. (N) Immunoblot of 143B and Bkid cell lysates. OB-cadherin and K-cadherin was upregulated, whereas Snail2 was downregulated. The ratios of phosphorylated to whole Akt for each sample are shown between the blots. The ratio of phosphorylated to whole p38MAPK, and the ratio of phosphorylated to whole ERK1/2 are also shown between each blot. Expression of MMP9 was diminished in Bkid cells, whereas that of MMP14, MMP15, and LPP was decreased. (O) Comparisons between Bkid and Bkid+PEDFmiR cell lysates. The amount of PEDF were reduced. The ratios of phosphorylated to whole Akt and phospho-p38MAPK to whole p38 are also shown between the blots. The phosphorylation level decreased upon PEDF knockdown. Snail2 was upregulated in Bkid+PEDFmiR, whereas K-cadherin was downregulated. (P) Zymography indicating that metalloprotease activity was increased upon PEDF knockdown. The upregulated MMP was mainly MMP9. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35174090), licensed under a CC-BY license. Not internally tested by R&D Systems.