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Human Cadherin-17 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1032

R&D Systems, part of Bio-Techne
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BAF1032

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cadherin‑17
Gln23-Met787
Accession # CAA58231

Specificity

Detects human Cadherin‑17 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Cadherin-8,rhE-Cadherin, rhP-Cadherin, and rhVE-Cadherin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cadherin-17 Biotinylated Antibody

Cadherin-17 antibody in Human Colon by Immunohistochemistry (IHC-P).

Cadherin‑17 in Human Colon.

Cadherin-17 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human Cadherin-17 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1032) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cadherin-17 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon array

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cadherin-17

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-17

Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa.

Alternate Names

Cadherin17, CDH17

Entrez Gene IDs

1015 (Human); 12557 (Mouse)

Gene Symbol

CDH17

UniProt

Additional Cadherin-17 Products

Product Documents for Human Cadherin-17 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Cadherin-17 Biotinylated Antibody

For research use only

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