HEK293 human embryonic kidney cell line transfected with either (A) human Calcium-sensing R/CaSR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Calcium-sensing R/CaSR Monoclonal Antibody (Catalog # MAB9608) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Calcium-sensing R/CaSR Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcium-sensing R/CaSR

Calcium-sensing receptor (CasR) is a plasma membrane G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (1). CasR is expressed in the parathyroid hormone-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (2). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (3). The full length of CasR has a large extracellular N-terminal domain, a central region of 7 transmembrane domains, and a long intracellular C-terminal domain. The amino acid 20-601 contains the extracellular domain with multiple glycosylation sites (4). Human CasR shares 96.1% and 96.5% aa sequence identity with mouse and rat CasR, respectively.

References

Geng, Y. et al. (2016) Elife 5:e13662.
Hendy, G. N. et al. (2000) Hum. Mutat. 16:281.
Brown, E. M. et al. (1993) Nature 366:575.
Garrett, J. E.et al. (1995) J. Biol. Chem. 270:12919.

Long Name

Calcium-sensing Receptor

Alternate Names

CAR, CaSR, EIG8, FHH, FIH, GPRC2A, HHC1, NSHPT, PCaR1

Entrez Gene IDs

846 (Human); 12374 (Mouse); 24247 (Rat)

Gene Symbol

CASR

UniProt

P41180-1

Additional Calcium-sensing R/CaSR Products

Product Documents for Human Calcium-sensing R/CaSR Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Calcium-sensing R/CaSR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Calcium-sensing R/CaSR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9608

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Calcium-sensing R/CaSR Antibodies »

Product Summary for Human Calcium-sensing R/CaSR Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Calcium-sensing R/CaSR Antibody

Detection of Calcium-sensing R/CaSR in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP by Flow Cytometry.