Human Calreticulin-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2927

R&D Systems, part of Bio-Techne
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AF2927
AF2927-SP
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BAF2927
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Calreticulin-2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Calreticulin-2
Thr20-Leu384
Accession # Q96L12

Specificity

Detects human Calreticulin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Calreticulin-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Calreticulin-2 Antibody

Detection of Calreticulin2 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of Calreticulin2 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Goat Anti-Human Calreticulin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2927, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human Calreticulin-2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: MCF‑7 human breast cancer cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Calreticulin-2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calreticulin-2

Calreticulin-2 is a testis-specific, soluble, lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca++ ion with high affinity, and the C-terminus binds Ca++ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin-2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin-2, respectively.

Alternate Names

CALR3, Calreticulin2, Crt2

Entrez Gene IDs

125972 (Human); 73316 (Mouse); 364529 (Rat)

Gene Symbol

CALR3

UniProt

Q96L12

Additional Calreticulin-2 Products

Product Documents for Human Calreticulin-2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Calreticulin-2 Antibody

For research use only

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