Human Calsyntenin-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5346

R&D Systems, part of Bio-Techne
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AF5346
AF5346-SP
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Biotin
BAF5346
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Calsyntenin-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Calsyntenin-1 isoform 2
Arg30-Thr851
Accession # AAH33902

Specificity

Detects human Calsyntenin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Calsyntenin-2 and rhCalsyntenin-3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Calsyntenin-1 Antibody

Detection of Human Calsyntenin-1 antibody by Western Blot.

Detection of Human Calsyntenin-1 by Western Blot.

Western blot shows lysates of human brain cortex and brain cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Calsyntenin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5346) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Calsyntenin-1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Calsyntenin-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain cortex and brain cerebellum tissue

Reviewed Applications

Read 1 review rated 5 using AF5346 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calsyntenin-1

Calsyntenin-1 (CST-1; also Alcadein-alpha) is a 140‑150 kDa member of the Alcadein family of molecules. It is expressed in the ER/Golgi and on the plasma membranes of almost all neurons. It is a calcium-binding protein that may regulate post-synaptic signaling and APP cleavage and gene activation. Notably, when cleaved in a manner similar to APP, its intracellular fragment antagonizes APP AICD gene activation. Mature human CST-1 is a 953 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 38‑265) in its extracellular region (aa 29‑859) and a 101 aa cytoplasmic tail. Extracellular cleavage occurs at Met825 Ala826, generating a soluble 115 kDa ECD. Isoforms involve a 78 aa substitution for aa 917‑981, plus deletions of aa 72‑81 and 507‑525. Over aa 30‑851, human CST-1 shares 92% aa identity with mouse CST-1.

Alternate Names

Alcadein-alpha, Calsyntenin1, CLSTN1, CS1, CSTN1, XB31alpha

Entrez Gene IDs

22883 (Human); 65945 (Mouse); 313717 (Rat)

Gene Symbol

CLSTN1

UniProt

AAH33902

Additional Calsyntenin-1 Products

Product Documents for Human Calsyntenin-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Calsyntenin-1 Antibody

For research use only

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