Human CaM Kinase II delta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4176
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 438422
Product Specifications
Immunogen
E. coli-derived recombinant human CaM Kinase II delta
Ala402-Lys478
Accession # Q13557
Ala402-Lys478
Accession # Q13557
Specificity
Detects endogenous human CaM Kinase II delta isoforms in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKII alpha, CaMKII beta, or CaMKII gamma.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CaM Kinase II delta Antibody
Detection of Human CaM Kinase II delta by Western Blot.
Western blot shows lysates of human heart, liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CaM Kinase IId Monoclonal Antibody (Catalog # MAB4176) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CaM Kinase IId isoforms at approximately 50 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human CaM Kinase II delta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human heart, liver and kidney tissue
Sample: Human heart, liver and kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CaM Kinase II delta
Calcium/calmodulin-dependent protein kinase type II delta (CaMKII delta) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and delta, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKII delta expression is ubiquitous and has been identified as the predominant CaMKII isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.
Long Name
Calcium/calmodulin-dependent Protein Kinase II delta Subunit
Alternate Names
CAMK2D
Gene Symbol
CAMK2D
UniProt
Additional CaM Kinase II delta Products
Product Documents for Human CaM Kinase II delta Antibody
Product Specific Notices for Human CaM Kinase II delta Antibody
For research use only
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