Human/Canine CD11b/Integrin alpha M Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1699
Key Product Details
Species Reactivity
Validated:
Human, Canine
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alphaM
Phe17-Asn1105
Accession # NP_001139280
Phe17-Asn1105
Accession # NP_001139280
Specificity
Detects human CD11b/Integrin alphaM in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Integrin alphaM and recombinant human Integrin alphaE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Canine CD11b/Integrin alpha M Antibody
Detection of CD11b/Integrin alphaM in Canine PBMCs by Flow Cytometry.
Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine CD11b/Integrin aM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Detection of CD11b/Integrin alpha M in PBMC monocytes cells by Flow Cytometry.
PBMC monocytes cells were stained with Goat Anti-Human/Canine CD11b/Integrin alpha M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.Applications for Human/Canine CD11b/Integrin alpha M Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells and canine peripheral blood mononuclear cells (PBMCs)
Sample: Human peripheral blood mononuclear cells and canine peripheral blood mononuclear cells (PBMCs)
Western Blot
0.1 µg/mL
Sample: Recombinant Human CD11b/Integrin alphaM
Sample: Recombinant Human CD11b/Integrin alphaM
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF1699 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
UniProt
Additional CD11b/Integrin alpha M Products
Product Documents for Human/Canine CD11b/Integrin alpha M Antibody
Product Specific Notices for Human/Canine CD11b/Integrin alpha M Antibody
For research use only
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