Human/Canine Integrin beta1/CD29 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1778
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Canine
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Functional Assay, Immunocytochemistry, Migration Assay, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta1 isoform 1A
Gln21-Asp728
Accession # P05556
Gln21-Asp728
Accession # P05556
Specificity
Detects human Integrin beta1/CD29 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Canine Integrin beta1/CD29 Antibody
Detection of Human Integrin beta1/CD29 by Western Blot.
Western blot shows lysates of MG-63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta1/CD29 at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Integrin beta1/CD29 by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin beta1/CD29 at approximately 198 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Canine Integrin beta1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Integrin beta1/CD29 in Canine PBMCs by Flow Cytometry.
Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Applications for Human/Canine Integrin beta1/CD29 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs), canine peripheral blood mononuclear cells (PBMCs), and canine mesenchymal stem cells
Sample: Human peripheral blood mononuclear cells (PBMCs), canine peripheral blood mononuclear cells (PBMCs), and canine mesenchymal stem cells
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed canine mesenchymal stem cells, human mesenchymal stem cells, and A549 human lung carcinoma cell line
Sample: Immersion fixed canine mesenchymal stem cells, human mesenchymal stem cells, and A549 human lung carcinoma cell line
Knockout Validated
Integrin beta1/CD29
is specifically detected in A549 human lung carcinoma parental cell line but is not detectable in
Integrin beta1/CD29 knockout A549 cell line.
Simple Western
10-20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Western Blot
1 µg/mL
Sample: MG‑63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Sample: MG‑63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
UniProt
Additional Integrin beta 1/CD29 Products
Product Documents for Human/Canine Integrin beta1/CD29 Antibody
Product Specific Notices for Human/Canine Integrin beta1/CD29 Antibody
For research use only
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