Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Canine NGFR/TNFRSF16 Antibody

Detection of Human NGF R/TNFRSF16 by Western Blot.

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NGF R/TNFRSF16 at approximately 65 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Detection of NGF R/TNFRSF16 in SH‑SY5Y Human Cell Line by Flow Cytometry.

SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

NGF R/TNFRSF16 in Canine Mesenchymal Stem Cells.

NGF R/TNFRSF16 was detected in immersion fixed canine mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Applications for Human/Canine NGFR/TNFRSF16 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: SHSY-5Y human cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed canine and human mesenchymal stem cells

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

2 µg/mL
Sample: SW480 human colorectal adenocarcinoma cell line
under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB367 in the following applications:

Immunocytochemistry (1 Review)
Immunohistochemistry (1 Review)
Immunohistochemistry-Frozen (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGFR/TNFRSF16

NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin-3 and Neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).

References

Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
Stemple, D.L. et al. (1992) Cell 71:973.
Morrison, S.J. et al. (1999) Cell 96:737.
Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
Cassiman, D. et al. (2001) Hepatology 33:148.

Long Name

Nerve Growth Factor Receptor

Alternate Names

CD271, NGF R, p75 NTR, TNFRSF16

Entrez Gene IDs

4804 (Human); 18053 (Mouse); 117089 (Rat)

Gene Symbol

NGFR

UniProt

P08138

Additional NGFR/TNFRSF16 Products

Product Documents for Human/Canine NGFR/TNFRSF16 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Canine NGFR/TNFRSF16 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Canine NGFR/TNFRSF16 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB367

Key Product Details

Species Reactivity

Validated:

Cited:

Applications