NS0 mouse myeloma cell line transfected with human Cannabinoid R1/CB1/CNR1 and eGFP was stained with and either (A) Mouse Anti-Human Cannabinoid R1/CB1/CNR1 Monoclonal Antibody (Catalog # MAB3834) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human Cannabinoid R1/CB1/CNR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: NS0 mouse myeloma cell line transfected with human Cannabinoid R1/CB1/CNR1 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cannabinoid R1/CB1/CNR1

Cannabinoid R1 (CB1R) is a 60 kDa 7TM protein that belongs to the family of G protein-coupled receptors, class A. CB1R is expressed in the central nervous system and upper GI tract, in contrast to CB2R which is expressed by hematopoietic cells. CB1R mediates the behavioral and gut motility effects of cannabinoids. Human CB1R exists in three alternately spliced forms which are distinguished by N-terminal substitutions or deletions. Human CB1R shares 97% amino acid sequence identity with mouse and rat CB1R.

Long Name

Cannabinoid Receptor 1

Alternate Names

CANN6, CannabinoidR1, CB-R, CB1, CB1A, CB1K5, CB1R, CNR1, SKR6R

Entrez Gene IDs

1268 (Human); 12801 (Mouse); 25248 (Rat)

Gene Symbol

CNR1

UniProt

NP_057167

Additional Cannabinoid R1/CB1/CNR1 Products

Product Documents for Human Cannabinoid R1/CB1/CNR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cannabinoid R1/CB1/CNR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Cannabinoid R1/CB1/CNR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3834

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Cannabinoid R1/CB1/CNR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Cannabinoid R1/CB1/CNR1 Antibody

Detection of Cannabinoid R1/CB1/CNR1 in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP by Flow Cytometry.