Detects human Cannabinoid R2/CB2/CNR2 in direct ELISAs. Stains human Cannabinoid R2/CB2/CNR2 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Cannabinoid R2/CB2/CNR2 Antibody

Detection of Cannabinoid R2/CB2/CNR2 in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human Cannabinoid R2/CB2/ CNR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Cannabinoid R2/CB2/CNR2 Monoclonal Antibody (Catalog # MAB36551) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Cannabinoid R2/CB2/CNR2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human Cannabinoid R2/CB2/CNR2 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cannabinoid R2/CB2/CNR2

Cannabinoid R2 (Cannabinoid Receptor 2), also called CB2 or CNR2, is a 7TM protein that belongs to the family of G protein-coupled receptors, class A. Cannabinoid R2 is expressed in the periphery, notably by hematopoietic cells, in contrast to Cannabinoid R1 which is expressed predominantly in the central nervous system and gut. Cannabinoid R2 on lymphocytes and macrophages binds cannabinoids and modulates cytokine secretion. With the exception of their divergent C‑terminal tails, human Cannabinoid R2 shares 82% amino acid sequence identity with mouse and rat Cannabinoid R2.

Long Name

Cannabinoid Receptor 2

Alternate Names

CannabinoidR2, CB2R, CNR2, CX5

Entrez Gene IDs

1269 (Human); 12802 (Mouse); 57302 (Rat)

Gene Symbol

CNR2

UniProt

NP_001832

Additional Cannabinoid R2/CB2/CNR2 Products

Product Documents for Human Cannabinoid R2/CB2/CNR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cannabinoid R2/CB2/CNR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Cannabinoid R2/CB2/CNR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB36551

Key Product Details

Species Reactivity

Validated:

Cited:

Applications