Human Carbonic Anhydrase IX Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2188
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
Functional Assay
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX (R&D Systems, Catalog # 2188-CA)
Pro59-Asp414
Accession # Q16790
Pro59-Asp414
Accession # Q16790
Specificity
Detects human Carbonic Anhydrase IX (CA9) in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with recombinant mouse (rm) CA9, rhCA12, and rhCA14 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Carbonic Anhydrase IX Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase IX (Catalog # 2188-CA)
Sample: Recombinant Human Carbonic Anhydrase IX (Catalog # 2188-CA)
Human Carbonic Anhydrase IX Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IX/CA9
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1 - 3). Topics in the CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.
References
- Pastorek, J. et al. (1994) Oncogene 9: 2877.
- Opavsky, R. et al. (1996) Genomics 33: 480.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
- Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Alternate Names
CA9, G250, MN, RCC
Gene Symbol
CA9
UniProt
Additional Carbonic Anhydrase IX/CA9 Products
Product Documents for Human Carbonic Anhydrase IX Biotinylated Antibody
Product Specific Notices for Human Carbonic Anhydrase IX Biotinylated Antibody
For research use only
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