Human Carboxylesterase 2/CES2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5657
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxylesterase 2/CES2
Gln27-Leu559
Accession # O00748
Gln27-Leu559
Accession # O00748
Specificity
Detects human Carboxylesterase 2/CES2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human CES1, recombinant mouse (rm) CES2, rmCES3, and rmCES5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Carboxylesterase 2/CES2 Antibody
Detection of Human Carboxylesterase 2/CES2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NCI-N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carboxylesterase 2/CES2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5657) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carboxylesterase 2/CES2 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Carboxylesterase 2/CES2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and NCI‑N87 human gastric carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and NCI‑N87 human gastric carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxylesterase 2/CES2
Alternate Names
CE-2, CES2, PCE-2
Gene Symbol
CES2
UniProt
Additional Carboxylesterase 2/CES2 Products
Product Documents for Human Carboxylesterase 2/CES2 Antibody
Product Specific Notices for Human Carboxylesterase 2/CES2 Antibody
For research use only
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