Human Carboxypeptidase A1/CPA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2856
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A1/CPA1
Lys17-Tyr419
Accession # P15085
Lys17-Tyr419
Accession # P15085
Specificity
Detects human Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant mouse CPA1 is observed and less than 10% cross-reactivity with recombinant human CPB1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Carboxypeptidase A1/CPA1 Antibody
Neutralization by Human CPA1 Antibody
Measured by its ability to neutralize Recombinant Human Carboxypeptidase A1/CPA1 (0.1 µg/mL, 2856-ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM). The Neutralization Dose (ND50) is <15 µg/mL.Applications for Human Carboxypeptidase A1/CPA1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN)
Sample: Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN)
Neutralization
Measured by its ability to neutralize Recombinant Human Carboxypeptidase A1/CPA1 (0.1 µg/mL, Catalog # 2856-ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM). The Neutralization Dose (ND50) is <15 µg/mL.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxypeptidase A1/CPA1
Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of human CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified rhCPA1 corresponds to the pro form which can be activated.
References
- Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 812, Academic Press, San Diego.
Alternate Names
CPA1
Gene Symbol
CPA1
UniProt
Additional Carboxypeptidase A1/CPA1 Products
Product Documents for Human Carboxypeptidase A1/CPA1 Antibody
Product Specific Notices for Human Carboxypeptidase A1/CPA1 Antibody
For research use only
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