Human CART Biotinylated Antibody

Name: Human CART Biotinylated Antibody
Brand: R&D Systems
SKU: BAF163

R&D Systems, part of Bio-Techne | Catalog # BAF163

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Product Datasheet / COA / SDS

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Conjugate

Catalog #

Unconjugated

AF163

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all CART Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CART
Gln28-Leu116
Accession # Q16568

Specificity

Detects human CART in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CART Biotinylated Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cortex)

Western Blot

0.1 µg/mL
Sample: Recombinant Human CART

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CART

CART, also known as cocaine and amphetamine-regulated transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations. In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment. There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region. There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist. These include a 5K, 48 aa peptide
(CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89). Human CART is 98% aa identical to both moyuse and rat CART (1-89). Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions. In addition, anatomical location is associated with differing lengths of CART. For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

References

Douglass, J. & S. Daoud (1996) Gene 169:241.
Douglass, J. et al. (1995) J. Neurosci. 15:247.
Adams, L.D. et al. (1999) Brain Res. 848:137.
Thim, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2722.
Bannon, A.W. et al. (2001) J. Pharmacol. Exp. Ther. 299:1021.
Kuhar, M.J. & L.L. Yoho (1999) Synapse 33:163.
Thim, L. et al. (1998) FEBS Lett. 428:263.

Long Name

Cocaine- and Amphetamine-regulated Transcript Protein

Alternate Names

CARTPT

Entrez Gene IDs

9607 (Human)

Gene Symbol