Human Caspr1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7548
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 753232
Product Summary for Human Caspr1 Antibody
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Caspr1
Trp20-Gly1280
Accession # P78357
Trp20-Gly1280
Accession # P78357
Specificity
Detects human Caspr1 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human Caspr2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Caspr1 Antibody
Caspr1 in IMR32 Human Cell Line.
Caspr1 was detected in immersion fixed IMR32 human neuroblastoma cell line using Mouse Anti-Human Caspr1 Monoclonal Antibody (Catalog # MAB7548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Caspr1 in A172 human glioblastoma cell line (Positive) & RT-4 human urinary bladder transitional cell papilloma cell line (Negative).
Caspr1 was detected in immersion fixed A172 human glioblastoma cell line (Positive) & RT-4 human urinary bladder transitional cell papilloma cell line (Negative) using Mouse Anti-Human Caspr1 Monoclonal Antibody (Catalog # MAB7548) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Caspr1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed IMR32 human neuroblastoma cell line, A172 human glioblastoma cell line (Positive) & RT-4 human urinary bladder transitional cell papilloma cell line (Negative)
Sample: Immersion fixed IMR32 human neuroblastoma cell line, A172 human glioblastoma cell line (Positive) & RT-4 human urinary bladder transitional cell papilloma cell line (Negative)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspr1
Long Name
Contactin Associated Protein-like 1
Alternate Names
CNTNAP, CNTNAP1, p190
Gene Symbol
CNTNAP1
UniProt
Additional Caspr1 Products
Product Documents for Human Caspr1 Antibody
Product Specific Notices for Human Caspr1 Antibody
For research use only
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