Human Cathepsin F Antibody

Name: Human Cathepsin F Antibody
Brand: R&D Systems
SKU: MAB2075

R&D Systems, part of Bio-Techne | Catalog # MAB2075

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 237208

View all Cathepsin F Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin F
Ala20-Asp484
Accession # NP_003784

Specificity

Detects human Cathepsin F in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin B, C, L, O, S, V, X/Z/P, recombinant mouse Cathepsin F, or H is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Cathepsin F Antibody

Cathepsin F in Human Heart.

Cathepsin F was detected in formalin fixed paraffin-embedded sections of human heart using 8 µg/mL Mouse Anti-Human Cathepsin F Monoclonal Antibody (Catalog # MAB2075) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cathepsin F Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin F, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Cathepsin F

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin F

Cathepsin F is a lysosomal cysteine protease that participates in protein degradation and has been implicated in tumor progression.

Alternate Names

CTSF

Entrez Gene IDs

8722 (Human); 56464 (Mouse)

Gene Symbol