Human Cathepsin F Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2075
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 237208
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin F
Ala20-Asp484
Accession # NP_003784
Ala20-Asp484
Accession # NP_003784
Specificity
Detects human Cathepsin F in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin B, C, L, O, S, V, X/Z/P, recombinant mouse Cathepsin F, or H is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Cathepsin F Antibody
Cathepsin F in Human Heart.
Cathepsin F was detected in formalin fixed paraffin-embedded sections of human heart using 8 µg/mL Mouse Anti-Human Cathepsin F Monoclonal Antibody (Catalog # MAB2075) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cathepsin F Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed paraffin-embedded sections of human heart
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin F, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin F, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Cathepsin F
Sample: Recombinant Human Cathepsin F
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin F
Additional Cathepsin F Products
Product Documents for Human Cathepsin F Antibody
Product Specific Notices for Human Cathepsin F Antibody
For research use only
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