Human Cathepsin S Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1183
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Immunohistochemistry, Western Blot
Cited:
Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331
Accession # P25774
Gln17-Ile331
Accession # P25774
Specificity
Detects human Cathepsin S in ELISAs and Western blots . In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human Cathepsin A, -B, -C, -D, E, L, V, and -X/Z/P is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Cathepsin S Biotinylated Antibody
Cathepsin S in Human Lymph Node.
Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cathepsin S Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Western Blot
0.1 µg/mL
Sample: Recombinant Human Cathepsin S (Catalog # 1183-CY)
Sample: Recombinant Human Cathepsin S (Catalog # 1183-CY)
Human Cathepsin S Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin S
Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.
References
- Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104, Academic Press, San Diego.
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
- Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
- Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
Alternate Names
CTSS
Entrez Gene IDs
1520 (Human)
Gene Symbol
CTSS
UniProt
Additional Cathepsin S Products
Product Documents for Human Cathepsin S Biotinylated Antibody
Product Specific Notices for Human Cathepsin S Biotinylated Antibody
For research use only
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