Human Cathepsin V Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10801
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 182021
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin V
Val18-Val334
Accession # O60911
Val18-Val334
Accession # O60911
Specificity
Detects human Cathepsin V in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) Cathepsin B, C, F, K, L, O, S, or Z is observed. In Western blots, less than 5% cross‑reactivity with rhCathepsin K and no corss-reactivity with rhCathepsin B, C, F, L, O, S, or Z is observed. This antibody specifically identifies the pro form but not the active form of Cathepsin V in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Cathepsin V Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin V (Catalog # 1080-CY), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin V (Catalog # 1080-CY), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Cathepsin V (Catalog # 1080-CY)
Sample: Recombinant Human Cathepsin V (Catalog # 1080-CY)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin V
Cathepsin V, also known as cathepsin L2 and cathepsin U, is a lysosomal cysteine protease expressed in the thymus, testis, and corneal epithelium. It may play a role in tumor progression since it is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues. Additionally, it may be involved in corneal physiology. The amino acid sequence of human Cathepsin V shares 99%, 83% and 76% identical with that of chimpanzee, dog and mouse/rat, respectively.
Alternate Names
Cathepsin L2, Cathepsin U, CTSL2
Entrez Gene IDs
1515 (Human)
Gene Symbol
CTSV
UniProt
Additional Cathepsin V Products
Product Documents for Human Cathepsin V Antibody
Product Specific Notices for Human Cathepsin V Antibody
For research use only
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