Human CCL14/HCC-1/HCC-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB324
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 256413
Product Specifications
Immunogen
E. coli-derived recombinant human CCL14/HCC‑1/HCC‑3
Thr20-Asn109
Accession # NP_116738
Thr20-Asn109
Accession # NP_116738
Specificity
Detects human CCL14/HCC‑1/HCC‑3 in direct ELISAs and Western blots. Detects both CCL14a and CCL14b. In Western blots, no cross‑reactivity with
recombinant human CCL1, 2, 3, 4, 5, 7, 8,
11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL2,
3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 24, 25, 28, or
recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human CCL14/HCC-1/HCC-3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL14a/HCC‑1 aa 20-93 (Catalog # 324-HC)
Sample: Recombinant Human CCL14a/HCC‑1 aa 20-93 (Catalog # 324-HC)
Reviewed Applications
Read 3 reviews rated 4.3 using MAB324 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL14/HCC-1/HCC-3
CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20‑93) is a 74 amino acid peptide that is also known as HCC-1 (Hemofiltrate CC Chemokine-1). It is a weak CCR1 agonist, however, an 8 amino acid N-terminal truncation (aa 28‑93) allows potent signaling through CCR1 and CCR5. CCL14b, also called HCC-3, is a 90 amino acid peptide (aa 20‑109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.
Alternate Names
C-C motif chemokine 14, chemokine (C-C motif) ligand 14, Chemokine CC-1/CC-3, chemokine CC-3, CKB1, FLJ16015, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, hemofiltrate CC chemokine 1, MCIF, member 14, NCC2CC-1, NCC-2CKb1, new CC chemokine 2, SCYA14CC-3, Small-inducible cytokine A14
Entrez Gene IDs
6358 (Human)
Gene Symbol
CCL14
UniProt
Additional CCL14/HCC-1/HCC-3 Products
Product Documents for Human CCL14/HCC-1/HCC-3 Antibody
Product Specific Notices for Human CCL14/HCC-1/HCC-3 Antibody
For research use only
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