Human CCL15/MIP-1 delta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF363
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL15/MIP-1 delta
Gln22-Ile113
Accession # Q16663
Gln22-Ile113
Accession # Q16663
Specificity
Detects human CCL15/MIP‑1 delta in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL15/MIP-1 delta Antibody
Chemotaxis Induced by CCL15/MIP‑1 delta and Neutralization by Human CCL15/MIP‑1 delta Antibody.
Recombinant Human CCL15/MIP-1d (363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP-1d (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP-1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363). The ND50 is typically 1-5 µg/mL.Applications for Human CCL15/MIP-1 delta Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL15/MIP-1 delta 92 aa (Catalog # 363-MG)
Sample: Recombinant Human CCL15/MIP-1 delta 92 aa (Catalog # 363-MG)
Neutralization
Measured by its ability to neutralize CCL15/MIP-1 delta-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL15/MIP-1 delta 92 aa.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL15/MIP-1 delta
References
- Wang, W. et al. (1998) J. Clinical Immunol. 18:214
Alternate Names
HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3
Entrez Gene IDs
6359 (Human)
Gene Symbol
CCL15
UniProt
Additional CCL15/MIP-1 delta Products
Product Documents for Human CCL15/MIP-1 delta Antibody
Product Specific Notices for Human CCL15/MIP-1 delta Antibody
For research use only
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