Human CCL17/TARC Antibody
R&D Systems, part of Bio-Techne | Catalog # AF364
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL17/TARC (R&D Systems, Catalog # 364-DN)
Ala24-Ser94
Accession # Q92583
Ala24-Ser94
Accession # Q92583
Specificity
Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TARC is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL17/TARC Antibody
Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody.
Recombinant Human CCL17/TARC (Catalog # 364-DN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/ TARC (0.01 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364). The ND50 is typically 0.3-1.5 µg/mL.CCL17/TARC in Human Spleen.
CCL17/TARC was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CCL17/TARC Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL17/TARC (Catalog # 364-DN)
Sample: Recombinant Human CCL17/TARC (Catalog # 364-DN)
Neutralization
Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL17/TARC.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL17/TARC
References
- Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
- Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
- Nomiyama, H. et al. (1997) Genomics 40:211.
Alternate Names
ABCD-2, SCYA17, TARC
Gene Symbol
CCL17
UniProt
Additional CCL17/TARC Products
Product Documents for Human CCL17/TARC Antibody
Product Specific Notices for Human CCL17/TARC Antibody
For research use only
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