Human CCL2/JE/MCP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB679R
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2B Clone # 23007R
Product Specifications
Immunogen
E. coli-derived recombinant human CCL2/JE/MCP‑1
Gln24-Thr99
Accession # P13500
Gln24-Thr99
Accession # P13500
Specificity
Detects human CCL2/JE/MCP‑1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL2/JE/MCP-1 Antibody
Human CCL2/JE/MCP‑1 ELISA Standard Curve.
Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC) was serially diluted 2-fold and captured by Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF279) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP-1 Antibody.
Recombinant Human CCL2/MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679R). The ND50 is typically 0.5-2.0 µg/mL.Applications for Human CCL2/JE/MCP-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC)
Sample: Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC)
Neutralization
Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 75 ng/mL Recombinant Human CCL2/JE/MCP‑1.
Human CCL2/JE/MCP-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB679R in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
UniProt
Additional CCL2/JE/MCP-1 Products
Product Documents for Human CCL2/JE/MCP-1 Antibody
Product Specific Notices for Human CCL2/JE/MCP-1 Antibody
For research use only
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