Human CCL20/MIP-3 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF360
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Neutralization
Cited:
Bioassay, ELISA Development, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL20/MIP-3 alpha
Ala27-Met96 (Asn95Asp)
Accession # P78556
Ala27-Met96 (Asn95Asp)
Accession # P78556
Specificity
Detects human CCL20/MIP-3 alpha in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL20/MIP-3 alpha Antibody
CCL20/MIP-3 alpha in Human PBMCs.
CCL20/MIP-3a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stumulated with LPS using Human CCL20/MIP-3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CCL20/MIP-3 alpha in Human Skin.
CCL20/MIP-3a was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human CCL20/MIP-3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CCL20/MIP-3 alpha and Neutralization by Human CCL20/MIP-3 alpha Antibody.
Recombinant Human CCL20/MIP-3a (360-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP-3a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL20/MIP-3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360). The ND50 is typically 0.2-1.0 µg/mL.Applications for Human CCL20/MIP-3 alpha Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with LPS
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with LPS
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin and human testis
Sample: Immersion fixed paraffin-embedded sections of human skin and human testis
Neutralization
Measured by its ability to neutralize CCL20/MIP-3 alpha-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 10 ng/mL Recombinant Human CCL20/MIP-3 alpha.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL20/MIP-3 alpha
CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.
CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.
References
- Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
- Hromas, R. et al. (1997) Blood 89:3315.
- Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Alternate Names
exodus-1, LARC, MIP-3 alpha, MIP3 alpha
Gene Symbol
CCL20
UniProt
Additional CCL20/MIP-3 alpha Products
Product Documents for Human CCL20/MIP-3 alpha Antibody
Product Specific Notices for Human CCL20/MIP-3 alpha Antibody
For research use only
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