Human CCL24/Eotaxin-2/MPIF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB343
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 61016
Product Specifications
Immunogen
E. coli-derived recombinant human CCL24/Eotaxin‑2/MPIF‑2
Val27-Cys119
Accession # AAB51135
Val27-Cys119
Accession # AAB51135
Specificity
Detects human CCL24/Eotaxin‑2/MPIF‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.04% cross-reactivity with recombinant human (rh) Eotaxin, rhMIP-1 alpha, and rhMCP-3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL24/Eotaxin-2/MPIF-2 Antibody
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Human CCL24/Eotaxin‑2 Antibody.
Recombinant Human CCL24/ Eotaxin-2 (Catalog # 343-E2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/ Eotaxin-2 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL24/ Eotaxin-2 Monoclonal Antibody (Catalog # MAB343). The ND50 is typically 0.2-0.8 µg/mL.Applications for Human CCL24/Eotaxin-2/MPIF-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL24/Eotaxin-2/MPIF-2 (Catalog # 343-E2)
Sample: Recombinant Human CCL24/Eotaxin-2/MPIF-2 (Catalog # 343-E2)
Neutralization
Measured by its ability to neutralize CCL24/Eotaxin-2/MPIF-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 100 ng/mL Recombinant Human CCL24/Eotaxin-2/MPIF-2.
Human CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL24/Eotaxin-2/MPIF-2
Eotaxin-2, also named MPIF-2 and Ck beta6, is a CC chemokine that is designated CCL24. Eotaxin-2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C‑terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin-2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP-3, CCL3/MIP-1 alpha, and CCL11/Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin-2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2, Eotaxin and CCL13/MCP-4 are mediated by the receptor CCR3.
References
- Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
- Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.
Alternate Names
Eotaxin-2, MPIF-2, MPIF2
Gene Symbol
CCL24
UniProt
Additional CCL24/Eotaxin-2/MPIF-2 Products
Product Documents for Human CCL24/Eotaxin-2/MPIF-2 Antibody
Product Specific Notices for Human CCL24/Eotaxin-2/MPIF-2 Antibody
For research use only
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