Human CCL28 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB717
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
ELISA Development, Functional Assay, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 62705
Product Specifications
Immunogen
E. coli-derived recombinant human CCL28
Ile23-Tyr127
Accession # Q9NRJ3
Ile23-Tyr127
Accession # Q9NRJ3
Specificity
Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11,
12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1,
2, 3, 4, 5, 6, 7, 9, 11, 19, 21, 22, 25, or recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL28 Antibody
Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody.
Recombinant Human CCL28 (Catalog # 717-VC) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB717). The ND50 is typically 15-45 µg/mL.Applications for Human CCL28 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL28 (Catalog # 717-VC)
Sample: Recombinant Human CCL28 (Catalog # 717-VC)
Neutralization
Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR10. The Neutralization Dose (ND50) is typically 15-45 µg/mL in the presence of 4 µg/mL Recombinant Human CCL28.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL28
Human CCL28 (CC chemokine ligand 28) is a 127 amino acid (aa) precursor protein with a putative 22 aa signal peptide that is cleaved to produce the 105 aa residue mature protein. Mature human and mouse CCL28 share 83% aa sequence identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression is highest in epithelial cells of normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. CCR10 (GPR2 orphan receptor) is the receptor for both CCL27/CTACK and CCL28.
References
- Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Alternate Names
CCL28, MEC, VIC
Gene Symbol
CCL28
UniProt
Additional CCL28 Products
Product Documents for Human CCL28 Antibody
Product Specific Notices for Human CCL28 Antibody
For research use only
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