Human CCL3/CCL4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2701
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 93342
Product Specifications
Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha
Ala27-Ala92
Accession # P10147
Ala27-Ala92
Accession # P10147
Specificity
Detects human CCL3/MIP-1 alpha in Western blots. In
direct ELISAs, 100% cross-reactivity with recombinant human (rh) CCL4/MIP-1 beta is
observed. Does not cross-react with rhCCL1, 2, 5, 7, 8, 11, 13, 14, 15, 16, 17,
18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 5, 6, 7,
CCL9/10MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human CCL3/CCL4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Sample: Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD) under non-reducing conditions only
Sample: Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD) under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/CCL4
References
- Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Long Name
CCL3/MIP-1 alpha and CCL4/MIP-1 beta Cross-reactive
Alternate Names
C-C motif chemokine;C-C motif chemokine 3;G0S19-1;LD78ALPHA;MIP1A;MIP-1-alpha;SCYA3
UniProt
Additional CCL3/CCL4 Products
Product Documents for Human CCL3/CCL4 Antibody
Product Specific Notices for Human CCL3/CCL4 Antibody
For research use only
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