Human CCL3/MIP-1 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB670
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Applications
Validated:
ELISA, Neutralization
Cited:
ELISA Development, Flow Cytometry, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 14215
Product Specifications
Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha
Specificity
Detects human CCL3/MIP-1 alpha.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL3/MIP-1 alpha Antibody
Chemotaxis Induced by CCL3/MIP-1 alpha and Neutralization by Human CCL3/MIP-1 alpha Antibody.
Recombinant Human CCL3/MIP-1a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP-1a (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB670). The ND50 is typically 3-10 µg/mL.Applications for Human CCL3/MIP-1 alpha Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB9849).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CCL3/MIP-1 alpha DuoSet ELISA Kit (Catalog # DY270) for convenient development of a sandwich ELISA or the Human CCL3/MIP-1 alpha Quantikine ELISA Kit (Catalog # DMA00) for a complete optimized ELISA.
Neutralization
Measured by its ability to neutralize CCL3/MIP-1 alpha-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 3-10 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL3/MIP-1 alpha isoform LD78a.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB670 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha
The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The
MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines.
Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.
References
- Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Alternate Names
G0S19-1, LD78a, MIP-1 alpha, MIP1 alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta
Entrez Gene IDs
Gene Symbol
CCL3
Additional CCL3/MIP-1 alpha Products
Product Documents for Human CCL3/MIP-1 alpha Antibody
Product Specific Notices for Human CCL3/MIP-1 alpha Antibody
For research use only
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