Human CCL3/MIP-1 alpha Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DMA00
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
Sensitivity
10 pg/mL
Assay Range
31.2-1000 pg/mL (Cell Culture Supernates)
46.9-1500 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
46.9-1500 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human CCL3/MIP-1 alpha Quantikine ELISA Kit
The Quantikine Human MIP-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MIP-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human MIP-1 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MIP-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 116 | 442 | 726 | 53.6 | 313 | 623 |
| Standard Deviation | 3.3 | 5.9 | 14.0 | 3.7 | 13.4 | 25.7 |
| CV% | 2.8 | 1.3 | 1.9 | 6.9 | 4.3 | 4.1 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 140 | 688 | 1084 | 50.7 | 306 | 613 |
| Standard Deviation | 12.5 | 60.4 | 55.4 | 5.9 | 15.1 | 37.8 |
| CV% | 8.9 | 8.8 | 5.1 | 11.6 | 4.9 | 6.2 |
Recovery for Human CCL3/MIP-1 alpha Quantikine ELISA Kit
The recovery of MIP-1 alpha spiked to different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 100 | 88-117 |
| Citrate Plasma (n=5) | 94 | 85-104 |
| EDTA Plasma (n=5) | 96 | 81-109 |
| Heparin Plasma (n=5) | 98 | 82-121 |
| Serum (n=5) | 95 | 87-113 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of MIP-1 alpha in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL3/MIP-1 alpha Quantikine ELISA Kit
Human CCL3/MIP-1 alpha ELISA Cell Culture Supernate Standard Curve
Human CCL3/MIP-1 alpha ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL3/MIP-1 alpha
MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.
The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.
Alternate Names
G0S19-1, LD78a, MIP-1 alpha, MIP1 alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta
Entrez Gene IDs
Gene Symbol
CCL3
Additional CCL3/MIP-1 alpha Products
Product Documents for Human CCL3/MIP-1 alpha Quantikine ELISA Kit
Product Specific Notices for Human CCL3/MIP-1 alpha Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.