Human CCL4/MIP-1 beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-271-NA
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Neutralization, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Lateral Flow, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Specificity
Detects human CCL4/MIP-1 beta in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL4/MIP-1 beta Antibody
Chemotaxis Induced by CCL4/MIP‑1 beta and Neutral-ization by Human CCL4/MIP‑1 beta Antibody.
Recombinant Human CCL4/MIP-1 beta (271-BME) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL4/MIP-1 beta (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA). The ND50 is typically 0.3-1.5 µg/mL.
CCL4/MIP‑1 beta in Human Alzheimer's Disease Brain.
CCL4/MIP-1 beta was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (hippocampus) using 15 µg/mL Goat Anti-Human CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CCL4/MIP‑1 beta in Human PBMCs.
CCL4/MIP-1 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Goat Anti-Human CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CCL4/MIP-1 beta Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with PHA and monensin
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with PHA and monensin
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (hippocampus)
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME)
Sample: Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME)
Neutralization
Measured by its ability to neutralize CCL4/MIP-1 beta-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 40 ng/mL Recombinant Human CCL4/MIP-1 beta.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF-271-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL4/MIP-1 beta
Alternate Names
Exodus-3, MIP-1 beta, MIP1 beta
Entrez Gene IDs
Gene Symbol
CCL4
Additional CCL4/MIP-1 beta Products
Product Documents for Human CCL4/MIP-1 beta Antibody
Product Specific Notices for Human CCL4/MIP-1 beta Antibody
For research use only
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