Human CCL8/MCP-2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF281
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
ELISA Development, ELISA Development (Detection)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL8/MCP-2 (R&D Systems, Catalog # 281-CP)
Gln24-Pro99
Accession # P80075
Gln24-Pro99
Accession # P80075
Specificity
Detects human CCL8/MCP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) HCC‑1, recombinant mouse (rm) MIP-1 alpha, rhMCP-1, and rhMCP-3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human CCL8/MCP-2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL8/MCP-2 (Catalog # 281-CP)
Sample: Recombinant Human CCL8/MCP-2 (Catalog # 281-CP)
Human CCL8/MCP-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using BAF281 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL8/MCP-2
MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.
Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Alternate Names
MCP-2, MCP2
Gene Symbol
CCL8
UniProt
Additional CCL8/MCP-2 Products
Product Documents for Human CCL8/MCP-2 Biotinylated Antibody
Product Specific Notices for Human CCL8/MCP-2 Biotinylated Antibody
For research use only
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