Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR2 PE-conjugated Monoclonal Antibody (Catalog # FAB151P) or (B) Mouse IgG_2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Detection of Human CCR2 by Flow Cytometry

Monocyte differentiation stages in the BM.Monocyte differentiation stages were characterized by CD45, CD34, CD33 and CD14 expression. HLA-DR and CCR2 expression were measured in each stage and expressed as the number of sites per cell. a/ example of FACs analysis for monocyte differentiation (red: myelo/monoblasts, blue: promonocytes, green: monocytes), and the HLA-DR and CCR2 expression levels in each stage (patient 31, see S1 Table); b/ individual values for HLA-DR expression (n = 10); c/ individual values for CCR2 expression (n = 10 for myelo/monoblasts, n = 11 for other cell populations). Wilcoxon comparisons. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0164489), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CCR2 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 6 reviews rated 4.3 using FAB151P in the following applications:

Flow Cytometry (6 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

UniProt

NP_001116868

Additional CCR2 Products

Product Documents for Human CCR2 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human CCR2 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Human CCR2 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB151P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

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Cited:

Label

Antibody Source

View all CCR2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CCR2 PE-conjugated Antibody

Detection of CCR2 in Human PBMCs by Flow Cytometry.