Human CD-M6PR Lumenal Domain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5320
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD-M6PR Lumenal Domain
Thr27-His185
Accession # P20645
Thr27-His185
Accession # P20645
Specificity
Detects human CD-M6PR Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross‑reactivity with recombinant mouse CD-M6PR is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CD-M6PR Lumenal Domain Antibody
Detection of Human CD-M6PR Lumenal Domain by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD-M6PR Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD-M6PR Lumenal Domain at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human CD-M6PR Lumenal Domain Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD-M6PR
The CD-M6PR (cation-dependent mannose 6-phosphate receptor; also CD-MPR) is a 44‑46 kDa member of the P-type lectin family of proteins. It is ubiquitously expressed, found internally, and transports newly formed mannose 6-phosphate bearing acid hydrolases from the secretory pathway to acidified lysosomes. Mature human CD-M6PR is a type I transmembrane glycoprotein that is 251 amino acids (aa) in length. It functions as a homodimer, and contains a 159 aa luminal region (aa 27‑185) plus a 67 aa cytoplasmic domain. The aa residues Gln66, Arg111, Glu133 and Tyr143 recognize carbohydrate; Asp103 interacts with divalent cation (Mn2+), increasing ligand affinity and receptor oligomerization. Over aa 27‑184, human CD-M6PR shares 90% aa identity with mouse CD-M6PR.
Long Name
Cation Dependent Mannose-6-Phosphate Receptor 46 kDa
Alternate Names
CD-MPR, CDM6PR, M6PR, MPR46, MPRD, SMPR
Gene Symbol
M6PR
UniProt
Additional CD-M6PR Products
Product Documents for Human CD-M6PR Lumenal Domain Antibody
Product Specific Notices for Human CD-M6PR Lumenal Domain Antibody
For research use only
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