Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # ICRF44

View all CD11b/Integrin alpha M Antibodies »

Product Specifications

Immunogen

Human rheumatoid synovial cells and monocytes

Specificity

Detects human CD11b/Integrin alphaM in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human CD11b/Integrin alpha M Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Immunohistochemistry

8-25 µg/mL
Sample: Myones, B. et al. (1988) J. Clin Invest. 82: 640.  This clone is not recommended for paraffin-embedded sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alphaM subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta2 subunit (CD18) to form the non-covalent heterodimer Integrin alphaM/ beta2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alphaM/ beta2 is expressedon granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alphaM/ beta2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement opsonized particles.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

Additional CD11b/Integrin alpha M Products

Product Documents for Human CD11b/Integrin alpha M Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD11b/Integrin alpha M Biotinylated Antibody

For research use only

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