Human CD11c Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1777
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunoprecipitation
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # ICRF 3.9
Product Specifications
Immunogen
Rheumatoid synovial fluid cells and human monocyte-derived fibronectin
Specificity
Detects human CD11c.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human CD11c Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood monocytes
Sample: Human whole blood monocytes
Immunoprecipitation
Hogg, N. et al. (1986) Eur. J. Immunol. 16:240.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using MAB1777 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11c
CD11c, also known as Integrin alphaX, is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta2, also known as CD18. The CD11c/CD18 complex, also called CR4 (complement receptor type 4) is expressed on macrophages, dendritic cells and hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells. It binds fibrinogen and has been reported to be a receptor for complement component iC3b (1‑3). The human CD11c extracellular domain (amino acids 20-1107) shares 70 - 76% amino acid sequence identity with mouse, rat and canine CD11c.
References
- Hogg, N. et al. (1986) Eur. J. Immunol. 16(3):240.
- Knapp, W.B. et al. eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
- Stacker, S.A. and T.A. Springer, J. Immunol. (1991) 146(2):648.
Alternate Names
CD11c, Integrin alpha X, ITGAX, p150,95 alpha
Gene Symbol
ITGAX
Additional CD11c Products
Product Documents for Human CD11c Antibody
Product Specific Notices for Human CD11c Antibody
For research use only
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