NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 PE‑conjugated Monoclonal Antibody (Catalog # FAB11331P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD133 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: NS0 mouse myeloma cell line transfected with human CD133

Reviewed Applications

Read 3 reviews rated 4.7 using FAB11331P in the following applications:

Flow Cytometry (3 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD133

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Entrez Gene IDs

8842 (Human); 19126 (Mouse)

Gene Symbol

PROM1

UniProt

O43490

Additional CD133 Products

Product Documents for Human CD133 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD133 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human CD133 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB11331P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD133 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD133 PE-conjugated Antibody

Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry.