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Human CD14 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3833

R&D Systems, part of Bio-Techne
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MAB3833-100
MAB3833-500
MAB3833-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

Binding Assay, ELISA Capture, ELISA Microarray Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 50040

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571

Specificity

Detects human CD14 in sandwich ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human CD14 Antibody

Human CD14 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CD14 Biotinylated Antibody (Catalog # BAF383)
  • Standard: Recombinant Human CD14 Protein (Catalog # 383-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB3833 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD14

CD14 occurs both as a secreted protein and as a GPI-linked membrane protein. It plays a critical role in lipopolysaccharide (LPS)-dependent signal transduction.

Alternate Names

CD14

Entrez Gene IDs

929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

Gene Symbol

CD14

UniProt

Additional CD14 Products

Product Documents for Human CD14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD14 Antibody

For research use only

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