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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgM Clone # ICRF29-2

Product Specifications

Immunogen

Human granulocytes

Specificity

Detects human CD15/Lewis X in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human CD15/Lewis X Alexa Fluor® 488-conjugated Antibody

Detection of CD15/Lewis X antibody in Human Peripheral Blood Granulocytes antibody by Flow Cytometry.

Detection of CD15/Lewis X in Human Peripheral Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CD15/Lewis X Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7368G, filled histogram) or isotype control antibody (Catalog # IC015G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD15/Lewis X Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human peripheral blood granulocytes

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD15/Lewis X

Lewis X (Lex) is a glycan structure (Gal beta1-4(Fuc alpha1-3)GlcNAc beta-) that forms the terminus of N-linked carbohydrates such as the antigenic epitope Stage-Specific Embryonic Antigen-1 (SSEA-1/CD15). It is widely expressed, can be found on glycoproteins, glycolipids and proteoglycans and is a blood group antigen. Lex is a marker for mouse embryonic stem and cancer cells, and is expressed on leukocytes, germ cells and neuronal stem cells in both mouse and human. Lex is thought to enhance cell adhesion, either directly or by promoting integrin activation.

Alternate Names

CD15, Lewis-X, LeX, SSEA-1

Additional CD15/Lewis X Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD15/Lewis X Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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