Human CD177 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3505

R&D Systems, part of Bio-Techne
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AF3505
AF3505-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CD177 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD177
Leu22-Gly407
Accession # AAH29167

Specificity

Detects human CD177 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD177 Antibody

Detection of CD177 antibody in Human Lymphocytes antibody by Flow Cytometry.

Detection of CD177 in Human Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Goat Anti-Human CD177 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3505, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human CD177 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood lymphocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human CD177 (Catalog # 3505-CD)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD177

CD177 is a polymorphic gene in the leukocyte antigen (Ly6) uPAR superfamily. It encodes at least two alleles of the neutrophilic membrane protein PRV1 (Polycythemic Rubra Vera protein 1) and NB1 (also named HNA-2a) that are glycosyl-phosphotidylinositol (GP1) anchored proteins of 58‑64 kDa. CD177 has two highly homologous cysteine-rich uPAR/Ly6 domains. The mRNA levels of CD177 is elevated under conditions where neutrophil counts are increased and is a useful marker for polycythemic vera. Mature human and mouse CD177 share 43% amino acid sequence identity. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (1-3).

References

  1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
  2. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
  3. Temerinac, S. et al. (2000) Blood 95:2569.

Long Name

Polycythemia Rubra Vera Protein 1

Alternate Names

CD177, HNA-2a, NB1 GP, PRV1

Entrez Gene IDs

57126 (Human); 68891 (Mouse)

Gene Symbol

CD177

UniProt

AAH29167

Additional CD177 Products

Product Documents for Human CD177 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD177 Antibody

For research use only

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