Human CD20 mFluor™ Violet 450-Conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4225MFV450
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 396444
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CD20
Met1-Pro297
Accession # P11836
Met1-Pro297
Accession # P11836
Specificity
Detects human CD20. Stains human CD20 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD20 mFluor™ Violet 450-Conjugated Antibody
![CD20 Antibody (396444) [mFluor Violet 450 SE]](https://resources.bio-techne.com/images/products/fab4225mfv450_mouse-cd20-mab-396444-mfluor-violet-450-se-flow-cytometry-532024105444..jpg "Detection of CD20 in PBMC lymphocytes by Flow Cytometry")
Detection of CD20 in PBMC lymphocytes by Flow Cytometry
PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD20 mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB4225MFV450) or (B) unstained . View our protocol for Staining Membrane-associated Proteins.Applications for Human CD20 mFluor™ Violet 450-Conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.1-1 µg/106 cells
Sample: PBMC lymphocytes
Sample: PBMC lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD20
CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.
Long Name
Cluster of Differentiation 20
Alternate Names
B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7
Entrez Gene IDs
Gene Symbol
MS4A1
UniProt
Additional CD20 Products
Product Documents for Human CD20 mFluor™ Violet 450-Conjugated Antibody
Product Specific Notices for Human CD20 mFluor™ Violet 450-Conjugated Antibody
mFluorTM is a trademark of AAT Bioquest, Inc.
For research use only
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