Human CD24 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5248
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 528807
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD24
Ser27-Gly59 (predicted)
Accession # P25063
Ser27-Gly59 (predicted)
Accession # P25063
Specificity
Detects human CD24 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD24 Antibody
CD24 in Human Colon.
CD24 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Human CD24 Monoclonal Antibody (Catalog # MAB5248) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CD24 in Human Ovarian Cancer Tissue.
CD24 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human CD24 Monoclonal Antibody (Catalog # MAB5248) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CD24 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon and human ovarian cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human colon and human ovarian cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4 using MAB5248 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD24
Alternate Names
CD24, CD24A
Gene Symbol
CD24
UniProt
Additional CD24 Products
Product Documents for Human CD24 Antibody
Product Specific Notices for Human CD24 Antibody
For research use only
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