Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1020N
Key Product Details
Validated by
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Glu22-Cys213
Accession # P01589
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody
Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with plate bound Human CD3 Monoclonal Antibody (Catalog # MAB100, 10 µg/ml), Human CD28 Monoclonal Antibody (Catalog # MAB342, 5 µg/ml) and 10 ng/mL Recombinant Human TGF- beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CD25/IL-2 R alpha Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1020N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody
Flow Cytometry
Sample: Human Treg cells (PBMC treated overnight with anti-human CD3 (10 μg/mL; Catalog # MAB100), anti-human CD28 (5 μg/mL; Catalog # MAB342), and TGF-beta (10 ng/mL; Catalog # 100-B).
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2R alpha
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional CD25/IL-2R alpha Products
Product Documents for Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody
Product Specific Notices for Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only