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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 24212

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589

Specificity

Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody

Detection of CD25/IL-2 R alpha antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with plate bound Human CD3 Monoclonal Antibody (Catalog # MAB100, 10 µg/ml), Human CD28 Monoclonal Antibody (Catalog # MAB342, 5 µg/ml) and 10 ng/mL Recombinant Human TGF- beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CD25/IL-2 R alpha Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1020N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human Treg cells (PBMC treated overnight with anti-human CD3 (10 μg/mL; Catalog # MAB100), anti-human CD28 (5 μg/mL; Catalog # MAB342), and TGF-beta (10 ng/mL; Catalog # 100-B).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD25/IL-2R alpha

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

Additional CD25/IL-2R alpha Products

Product Documents for Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD25/IL-2R alpha Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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