Human CD25/IL-2R alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2231R
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 22722R
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589
Glu22-Cys213
Accession # P01589
Specificity
Detects human CD25/IL-2 R alpha in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CD25/IL-2R alpha Antibody
Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody.
Recombinant Human IL-2 (202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB2231R). The ND50 is typically 0.2-1.2 µg/mL.Applications for Human CD25/IL-2R alpha Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 0.2-1.2 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA
Entrez Gene IDs
Gene Symbol
IL2RA
UniProt
Additional CD25/IL-2R alpha Products
Product Documents for Human CD25/IL-2R alpha Antibody
Product Specific Notices for Human CD25/IL-2R alpha Antibody
For research use only
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